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Figure 2. Insecticide resistance distribution of dengue vectors in Laos. (This map also shows result from the ARBOVEC project, see previous IPL annual reports for Characterization of genetic markers for insecticide resistance in Ae. aegypti from Laos (LECA – CNRS)

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: Figure 1. Map showing selected provinces for insecticide resistance monitoring in Laos.

: Figure 2. Insecticide resistance distribution of dengue vectors in Laos. (This map also shows result from the ARBOVEC project, see previous IPL annual reports for Characterization of genetic markers for insecticide resistance in Ae. aegypti from Laos (LECA – CNRS)

: Table 1. Resistance status of Aedes aegypti composite population (MetaLao) on larvae to temephos, deltamethrin, permethrin and, DDT

: Table 2. Resistance status of Aedes aegypti composite population (MetaLao) on adults to deltamethrin, permethrin, malathion and, DDT.

: Figure 3: Mortality rates of mosquitoes to the different insecticide over the generations (Fisher test: P<0,05 * compared with G2).

: Figure 4. Cross resistance test. A GLMer (binomial family) was performed to analyze these data. Each selected-line is identified by a different color. Different letters indicate significant mortality differences between lines for each insecticide tested.

: Figure 5: Allelic frequency of kdr mutations (V1016G and F1534C) for the different lineages (Fisher test: P<0.001 ***; P<0.01 **; P<0.05 *). NS: Not Selected.

: Figure 7: DNAg relative quantity variation for each gene. The black line represent the average CNV for the susceptible Bora strain.

: Figure 8. Primer design strategy adopted for developing a new PCR assay to genotype the presence/absence of a genomic amplification associated with insecticide resistance.

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